RNase R GMP-grade (20 U/μL)

Product description

Ribonuclease R (RNase R) is a magnesium-dependent 3'→5' exoribonuclease that can digest essentially all linear RNAs, but does not digest lariat or circular RNA structures, or double-stranded RNA with 3' overhangs shorter than 7 nucleotides. As such, this enzyme is ideally suited to the study of lariat RNA produced by traditional splicing, as well as circRNAs which arise through back-splicing. By removing linear RNAs from cellular or RNA extracts, RNase R greatly facilitates the identification of circular species through RNA-sequencing. This enables researchers to probe the landscape of splicing events with greater depth.

This product is produced in accordance with GMP process requirements and provided in liquid form.

Specifications

Storage

This product should be stored at -25~-15℃ for  two years.

Instructions

Experimental methods

1. The following reaction system was formulated in a sterile micro-centrifuge tube:

^*According to the specific purpose of experiments, it is necessary to prepare the corresponding reaction buffer. You can consider buying 14616ES(10×RNase R Reaction Buffer GMP-grade) to use together.

2. Reaction condition：37℃ for 10 min to 30 min.
3. Inactivation condition: incubation at 70℃ for 10 min can inactivate the enzyme.

Notes

1. Please operate with lab coats and disposable gloves，for your safety.

Ver.EN20240407