Recombinant Human GP100(HLA-A*02:01)(His-Avi Tag)
Recombinant Human KRAS G12V(HLA-A*03:01)(His-Avi Tag)
Recombinant Human AFP(HLA-A*02:01)(His-Avi Tag)
Recombinant Human NY-ESO-1(HLA-A*02:01)(His-Avi Tag)
Recombinant Human HLA-E*01:03 Complex(His-Avi Tag)
AFP (alpha-Fetoprotein), is albuminoid superfamily protein that is synthesized in the fetus primarily by the liver, yolk sac, and tissues of gastrointestinal origin. It is one of the earliest markers of the hepatocyte lineage. AFP acts as a carrier protein for steroids, bilirubin, fatty acids, retinoids, and flavonoids. Altered levels of both fetal and maternal AFP have been associated with hypothyroidism, autoimmune disorders, and heart defects. Elevated AFP levels are also coincident with liver, stomach, and germ cell cancers. Low maternal serum AFP levels are associated with a higher incidence of Down syndrome, whereas higher levels are associated with spina bifida and anencephaly.
产品性质
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别名（Synonyms） |
Alpha-fetoprotein, Alpha-1-fetoprotein, Alpha-feto, HPAFP, AFPD, FETA, MHC |
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Uniprot No. |
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表达区间及表达系统（Source） |
Recombinant Human AFP(HLA-A*02:01) Protein is expressed from HEK293 Cells with His tag and Avi tag at the C-terminal. It contains Gly25-Thr305 (HLA-A*02:01), Ile21-Met119 (B2M) and FMNKFIYEI peptide. |
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分子量(Molecular Weight) |
Approximately 50.6 kDa. Due to glycosylation, the protein migrates to 53-60 kDa based on Tris-Bis PAGE result. |
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外观(Physical Appearance） |
Sterile Filtered White lyophilized (freeze-dried) powder. |
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纯度（Purity） |
> 95% as determined by SDS-PAGE and HPLC |
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活性（Activity） |
ELISA Data: Immobilized Human AFP (HLA-A*02:01) Complex, His Tag at 2 μg/mL (100 μL/well) on the plate. Dose response curve for Anti-AFP (HLA-A*02:01) Antibody, hFc Tag with the EC50 of 8.2 ng/mL determined by ELISA. |
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内毒素（Endotoxin） |
< 1.0 EU per 1μg of the protein by the LAL method. |
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制剂（Formulation） |
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
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复溶（Reconstitution） |
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL` is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water. |
运输与保存方法
冰袋运输。-20℃至-80℃保存，一年有效期。
复溶后，-20 至 -80°C，在未开封状态下保存 3-6 个月。复溶后，2-8°C 保存 2-7 天。
建议第一次使用时分装冻存，避免反复冻融。
注意事项
1. 避免反复冻融。
2. 为了您的安全和健康，请穿实验服并戴一次性手套操作。
3. 本产品仅作科研用途！
产品数据
Tris-Bis PAGE
HB220706
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