Recombinant Mouse M-CSF Protein,His Tag

Synonyms

CSF-1, MGI-IM, MCSF, M-CSF, MGC31930

Uniprot No.

Q3U4F9

Source

Recombinant Mouse M-CSF is expressed from Yeast with N-His. It contains Lys33-Glu262.

Molecular Weight

The recombinant mouse CSF1 comprises 230 amino acids and has a predicted molecular mass of 26 kDa. The apparent molecular mass of the protein is approximately 30 kDa in SDS-PAGE under reducing conditions due to glycosylation

Purity

> 95% as determined by SDS-PAGE.

Endotoxin

<1.0 EU per 1μg of the protein by the LAL method.

Formulation

Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4,with 1%BSA.

Reconstitution

1. We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile PBS, pH 7.4, with 0.1% BSA to a concentration of 0.1-1.0 mg/mL.

2.Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C.

3.Further dilutions should be made in PBS, which must contain carrier proteins, such as 0.1% BSA, 10% FBS, 5% HSA, 5% trehalose, one of four options.