Human LAG-1/CCL4L1

Accession

Q8NHW4

GeneID

388372

Source

E.coli-derived Human CCL4L1 protein,Ala24-Asn92.

Molecular Weight

Approximately7.8 kDa

AA Sequence

APMGSDPPTA CCFSYTARKL PRNFVVDYYE TSSLCSQPAV VFQTKRGKQV CADPSESWVQ EYVYDLELN

Tag

None

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Purity

> 97% by SDS-PAGE and HPLC analyses.

Biological Activity

Fully biologically active when compared to standard. The ED₅₀ as determined by a cell proliferation assay using human CCR5 transfected murine BaF3 cells is less than 2.0 ng/mL, corresponding to a specific activity of > 5.0 × 10⁵ IU/mg.

Endotoxin

<0.1 EU/μg of protein as determined by LAL method.

Formulation

Lyophilized from a 0.2 μm filtered concentrated solution in PBS.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.