Recombinant Human MIP-1α/CCL3重组人MIP-1α/CCL3

Product Name

Cat#

Size

Recombinant Human Macrophage Inflammatory Protein-1 alpha/CCL3 (Human MIP-1α/CCL3)

90950ES08

5 μg

90950ES20

20 μg

90950ES50

50 μg

90950ES60

100 μg

90950ES76

500 μg

Synonyms

G0/G1 switch regulatory protein 19-1, MIP-1-alpha, PAT 464.1, SIS-beta, Small-inducible cytokine A3,Tonsillar lymphocyte LD78 alpha protein

Accession

P10147

GeneID

6348

Source

E.coli-derived Human CCL3,Ala23-Ala94.

Molecular Weight

Approximately 7.8 kDa.

AA Sequence

ASLAADTPTA CCFSYTSRQI PQNFIADYFE TSSQCSKPGV IFLTKRSRQV CADPSEEWVQ KYVSDLELSA

Tag

None

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Purity

> 96% by SDS-PAGE and HPLC analyses.

Biological Activity

Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human monocytes is in a concentration range of 1.0-10 ng/mL.

Endotoxin

<  1.0 EU per 1μg of the protein by the LAL method.

Formulation

Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 100 mM NaCl.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions.