pAc5.1/V5-His A

别名：pAc5.1/V5-HisA

质粒类型：果蝇细胞表达载体

启动子：pAC5

克隆方法：多克隆位点，限制性内切酶

载体大小：5375 bp

5' 测序引物及序列：AC5:5'd[ACACAAAGCCGCTCCATCAG]3'

载体标签：V5 Epitope Tag, His Tag (6x)

载体抗性：Ampicillin (氨苄青霉素)

备注：

Constitutive insect cell expression. Can use pCoHygro or pCoBlast for selection.

The DES?-Constitutive Kit provides the vector pAc5.1/V5-His for high-level constitutive expression of recombinant proteins and a choice of Blasticidin (pCoBlast) or hygromycin (pCoHygro) selection. The vector offers the strong, constitutive promoter from the Drosophila actin 5C gene. The pAc5.1/V5-His vector also offers the following features:
Small size (5.4 kb) to improve DNA yields and increase subcloning efficiency.
C-terminal V5 epitope tag for rapid detection with Invitrogens Anti-V5 Antibody.
C-terminal 6xHis tag for simple purification of recombinant fusion proteins using nickel-chelating resin.

To facilitate cloning, a set of three vectors-A, B, and C-is provided. Each vector has the multiple cloning site in a different reading frame relative to the coding sequence of the C-terminal tag.