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pAc5.1/V5-His/LacZ
- 品牌:长沙艾碧维
- 货号: HG-VGI0070
- CAS编号:
- 产地:长沙
- 供应商报价: 面议
- 长沙艾碧维生物科技有限公司 更新时间:2024-03-13 13:58:29
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- 详细介绍
- 产品参数
质粒类型:果蝇细胞表达载体
启动子:pAC5
克隆方法:多克隆位点,限制性内切酶
载体大小:8433 bp
5' 测序引物及序列:AC5:5'd[ACACAAAGCCGCTCCATCAG]3'
载体标签:V5 Epitope Tag, His Tag (6x)
载体抗性:Ampicillin (氨苄青霉素)
载体介绍The DES®-Constitutive Kit provides the vector pAc5.1/V5-His for high-level constitutive expression of recombinant proteins and a choice of Blasticidin (pCoBlast) or hygromycin (pCoHygro) selection. The vector offers the strong, constitutive promoter from the Drosophila actin 5C gene. The pAc5.1/V5-His vector also offers the following features:
Small size (5.4 kb) to improve DNA yields and increase subcloning efficiency
C-terminal V5 epitope tag for rapid detection with Invitrogens Anti-V5 Antibody
C-terminal 6xHis tag for simple purification of recombinant fusion proteins using nickel-chelating resin
To facilitate cloning, a set of three vectors-A, B, and C-is provided. Each vector has the multiple cloning site in a different reading frame relative to the coding sequence of the C-terminal tag.
- 主要用途
- 果蝇细胞表达系统
- pAc5.1/V5-His A
- pAc5.1/V5-His B
- pAc5.1/V5-His C
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