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武汉NeweastBio现货/Ran-GTP 小鼠单抗
- 品牌:美国NewEast Biosciences
- 货号: 26915
- CAS编号:
- 产地:武汉
- 供应商报价: 面议 (市场参考价:¥ 4800)
- 武汉费斯德生物科技有限公司 更新时间:2023-04-16 23:55:39
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- 详细介绍
货号: 26915 规格: 30 µL 基因符号: RAN 描述: Anti-Ran-GTP Mouse Monoclonal Antibody 背景介绍: Ran (Ras-related nuclear protein) is a member of the Ras-superfamily GTPases. Ran is involved in the transport of proteins across the nuclear envelope, as well as in microtubule organization during mitosis. 免疫原: Recombinant full length protein of active Ran. 经过测试的应用: IP, IHC, IF 推荐稀释比:
IP: 1 µg for 1~2 mg total cellular proteins
IHC, IF: 1:50-1:250浓度: 1 mg/ml 宿主: mouse 形式: Liquid 克隆性: Monoclonal 亚型: IgG 纯度: Purified from ascites 防腐剂: No 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol 种属反应性: Anti-Ran-GTP monoclonal antibody recognizes active Ran of vertebrates. 储存条件: Store at -20°C. Avoid freeze/thaw cycles 免疫沉淀/蛋白质印迹:
Ran Activation Assay. Purified Ran proteins were loaded as a control (lanes 1) or immunoprecipitated after treated with GDP (lane 2) or GTPγS (lane 3). Immunoprecipitation was done with the anti-Ran-GTP monoclonal antibody (Cat. No. 26915). Immunoblot was with an anti-Ran polyclonal antibody.- 主要用途
- GTP酶是GTP/GDP结合蛋白。被细胞表面受体(GPCR)激活后, 它能调节下游效应器的功能。它可以结合GTP、水解GTP,因此在激活的GTP结合状态和非激活的GDP结合状态之间切换。它具有内在的GTP酶活性,可将GTP水解为GDP,从而使G蛋白失活。我们精心筛选的小鼠单克隆抗体能捕捉到与GTP结合的具有活性的GTP酶。它们已被用于通过免疫组织化学监测细胞和组织中 G 蛋白的活化和分布。