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武汉NeweastBio现货/Ral-GTP 小鼠单抗
- 品牌:美国NewEast Biosciences
- 货号: 26913
- CAS编号:
- 产地:美国
- 供应商报价: 面议 (市场参考价:¥ 4800)
- 武汉费斯德生物科技有限公司 更新时间:2023-04-16 23:55:39
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- 同类产品活性单抗(23件)
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- 详细介绍
货号: 26913 规格: 30 µL 基因符号: Ral 描述: Anti-Ral-GTP Mouse Monoclonal Antibody 背景介绍: The Ras-like small G proteins, RalA and RalB, are important components of Ras signaling pathways, implicated in the initiation and maintenance of tumorigenic transformation, as well as vesicle transport, apoptosis, transcription, cell migration, and cell proliferation. 免疫原: Recombinant full length protein of active RalB. 经过测试的应用: IP, IHC, IF 推荐稀释比:
IP: 1 µg for 1~2 mg total cellular proteins
IHC, IF: 1:50-1:250浓度: 1 mg/ml 宿主: Mouse 形式: Liquid 克隆性: Monoclonal 亚型: IgG 纯度: Purified from ascites 防腐剂: no 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol 种属反应性: Anti-Ral-GTP monoclonal antibody recognizes active RalA and RalB of vertebrates. 储存条件: Store at -20°C. Avoid repeated freezing and thawing 免疫沉淀/蛋白质印迹:
Ral activation assay. Purified RalB proteins were immunoprecipitated after treated with GDP (lane 1) or GTPγS (lane 2). Immunoprecipitation was done with the anti-Ral-GTP monoclonal antibody (Cat. No. 26913). Immunoblot was with an anti-RalB rabbit polyclonal antibody (Cat. No. 21033).- 主要用途
- GTP酶是GTP/GDP结合蛋白。被细胞表面受体(GPCR)激活后, 它能调节下游效应器的功能。它可以结合GTP、水解GTP,因此在激活的GTP结合状态和非激活的GDP结合状态之间切换。它具有内在的GTP酶活性,可将GTP水解为GDP,从而使G蛋白失活。我们精心筛选的小鼠单克隆抗体能捕捉到与GTP结合的具有活性的GTP酶。它们已被用于通过免疫组织化学监测细胞和组织中 G 蛋白的活化和分布。