Automated multiprotein complex production

A group of Scientists in EMBL (Europe Molecular Biology Laboratory) has developed a fully automated protein complex production system from cDNA, based on Tecan Freedom EVO workstation. Structural and functional studies of many multiprotein complexes depend on recombinant-protein overexpression. Rapid revision of expression experiments and diversification of the complexes are often crucial for success of these projects; therefore, automat ion is increasingly indispensable.We introduce Acembl, a versatile and automatable system for protein-complex expression in Escherichia coli that uses recombineering to facilitatemult igene assembly and diversification.We demonstrated protein-complex expression using Acembl, including production of the complete prokaryotic holotranslocon. Freedom EVO®全自动化液体处理工作站