深度解析｜新型高通量厌氧发酵技术如何重塑益生菌研发

益生菌是活细菌，对人体具有促进健康和发挥生物功能的作用。它们通常用于增加肠道所需微生物的数量，譬如在抗生素治疗后促进肠道菌群再生。这就是益生菌或益生菌营养补充剂市场大幅度增长的原因之一^[1]。人体肠道菌群及其健康促进作用的研究对营养产业尤为重要。因此，完全有必要对厌氧或微量需氧培养技术进行科学研究，比如在类似这些微生物的生长条件下培养益生菌。

菌种：两歧双歧杆菌

实验方案：pH调控下的分批发酵&补料分批发酵（气体出口外接第三方O2传感器）

实验条件：温度37 °C，振摇速度600 rpm，N₂=30 mL/min，圆孔板

实验结果

菌种：干酪乳杆菌

三种培养方案：pH调控下的分批发酵和补料分批发酵（分别7.5 h、10 h开始补料）

培养条件：T=37 °C，振摇=600 rpm，N₂=30 mL/min

实时在线检测实验结果

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