Recombinant Human LAG3/CD223(His Tag)

Uniprot No.

P18627-1

表达区间及表达系统（Source）

HEK293 Cells-derived Human LAG3 / CD223 Protein, Leu23-Gly434 with His tag at the C-terminal

分子量(Molecular Weight)

Approximately 45.5 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Tris-Bis PAGE result.

外观(Physical Appearance）

Sterile Filtered White lyophilized (freeze-dried) powder.

纯度（Purity）

> 95% as determined by SDS-PAGE and HPLC

活性（Activity）

1.ELISA Data: Immobilized Human LAG3, His Tag at 0.5 μg/ml (100 μL/Well). Dose response curve for Anti-LAG3 Ab., hFc Tag with the EC₅₀ of 0.17 μg/mL determined by ELISA.

2.ELISA Data: Immobilized Human LAG3, His Tag at 1μg/mL (100 μL/Well) on the plate. Dose response curve for Human FGL1, hFc Tag with the EC₅₀ of 2.3 μg/mL determined by ELISA.

内毒素（Endotoxin）

< 1.0 EU per 1μg of the protein by the LAL method.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.

复溶（Reconstitution）

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.