Recombinant Rat Prolactin

Synonyms

Mammotropin, Luterotropic hormone, Lutetropin

Accession

P01237

GeneID

24683

Source

E.coli-derived rat Prolactin protein, Leu30-Cys226.

Molecular Weight

Approximately 22.6 kDa.

AA Sequence

LPVCSGGDCQ TPLPELFDRV VMLSHYIHTL YTDMFIEFDK QYVQDREFIA KAINDCPTSS LATPEDKEQA QKVPPEVLLN LILSLVHSWN DPLFQLITGL GGIHEAPDAI ISRAKEIEEQ NKRLLEGIEK IISQAYPEAK GNEIYLVWSQ LPSLQGVDEE SKDLAFYNNI RCLRRDSHKV DNYLKFLRCQ IVHKNNC

Tag

None

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Purity

>98% by SDS-PAGE and HPLC analyses.

Biological Activity

The ED₅₀ as determined by a cell proliferation assay using rat Nb2-11 cells is less than 1.0 ng/mL, corresponding to a specific activity of > 1.0 × 10⁶ IU/mg. Fully biologically active when compared to standard.

Endotoxin

< 0.1 EU per 1μg of the protein by the LAL method.

Formulation

Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions.