Determination of folic acid by capillary electrophor

A rapid and simple method is presented for the determination of folic acid (FA) by capillary electrophoresis (CE) with chemiluminescence (CL)detection. This method was based on enhance effect of FA on the CL reaction between luminol and BrO− in alkaline aqueous solution. Optimal separation and determination was obtained with an electrophoretic buffer of 35mM sodium borate (pH 9.4) containing 0.8mM luminol, and an oxidizer solution of 1.6mM NaBrO in 100mM NaCO3 buffer solution (pH 12.0). Under the optimal conditions, the determination of FA was achieved in less than 20 min, and the detection limitwas 2.0×10−8 M(S/N= 3). The relative standard deviations (RSDs) on peak area and migration time were in the 1.5 and 1.1%, respectively. The present CECL method was applied to the determination of FA in commercial pharmaceutical tablets, apple juices and human urine. GX毛细管电泳仪(紫外检测)CL1020