Rabbit Anti-WAF1抗体说明书

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【导读】RabbitAnti-WAF1抗体说明书 RabbitAnti-WAF1抗体说明书FROM:ABCAMCat.Number: Rs-0741RQuantitysize: 0...

RabbitAnti-WAF1抗体说明书

FROM:ABCAM

Cat.Number: Rs-0741R

Quantitysize: 0.1ml （dilutewithpH7.4 0.01MPBSordiluentofantibody）

Background:Thisgeneencodesapotentcyclin-dependentkinaseinhibitor.Theencodedproteinbindstoandinhibitstheactivityofcyclin-CDK2or-CDK4complexes,andthusfunctionsasaregulatorofcellcycleprogressionatG1.Theexpressionofthisgeneistightlycontrolledbythetumorsuppressorproteinp53,throughwhichthisproteinmediatesthep53-dependentcellcycleG1phasearrestinresponsetoavarietyofstressstimuli.Thisproteincaninteractwithproliferatingcellnuclearantigen（PCNA）,aDNApolymeraseaccessoryfactor,andplaysaregulatoryroleinSphaseDNAreplicationandDNAdamagerepair.ThisproteinwasreportedtobespecificallycleavedbyCASP3-likecaspases,whichthusleadstoadramaticactivationofCDK2,andmaybeinstrumentalintheexecutionofapoptosisfollowingcaspaseactivation.Twoalternativelysplicedvariants,whichencodeanidenticalprotein,havebeenreported.Twofamiliesofcyclindependentkinaseinhibitors（CKIs）havebeenidentified.Thep21WAF1/Cip1familyinhibitsallkinasesinvolvedintheG1/Stransition.Thep16INK4afamilyinhibitsCdk4andCdk6specifically.

Specificity: ·anti-WAF1isarabbitmonoclonalantibodyunconjugated

·specificforWAF1of hu,ratandmo

·epitopemappingneartheaminoterminusofWAF1of

hu,ratandmoorigin

·useforwesternblotting,elisa,immunoprecipitation

andimmunohistochemistry

· ProteinAaffinitychromatographypurification,purity:>95%

·Isotype:IgG

·molwt:82kDa

Application: ·Westernblotting:1:100-500

·Immunohistochemistry:1:100-500

·ELISA:1:500-1000

· Optimalworkingdilutionsmustbedeterminedbytheenduser.

Storage: Storeat–20ºCforoneyear.Avoidrepeatedfreeze/thawcycles.Thelyophilizedantibodyisstableatroomtemperatureforatleastonemonthandforgreaterthanayearwhenkeptat-20ºC.WhenreconstitutedinsterilepH7.40.01MPBSordiluentofantibodytheantibodyisstableforatleasttwoweeksat2-4ºC.

ImportantNote: Thisproductassuppliedis intendedforresearchuseonly,notforuseinhuman,therapeuticordiagnosticapplications.

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2004-07-13 09:31:27

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