Recombinant SARS-COV-2 Spike RBD(Omicron XBB) Protein, His Tag

别名

S protein RBD; S glycoprotein RBD; Spike protein RBD

Accession

QHD43416.1

表达区间及表达系统

Recombinant SARS-CoV-2 Spike RBD (Omicron XBB) Protein is expressed from Expi293 with His tag at the C-terminal. It contains Arg319-Phe541(G339H, R346T, L368I, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, V445P, G446S, N460K, S477N, T478K, E484A, F486S, F490S, Q498R, N501Y, Y505H).

分子量

Approximately 26.21 kDa. Due to glycosylation, the protein migrates to 35-45 kDa based on Tris-Bis PAGE result.

纯度

> 95% as determined by SDS-PAGE and HPLC.

活性

ELISA Data：Immobilized SARS-CoV-2 Spike RBD (Omicron XBB) at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 27.0ng/ml determined by ELISA.

内毒素

< 1.0 EU per 1μg of the protein by the LAL method.

制剂

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).

复溶

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.