Recombinant Human Necti4 (hFc Tag)

Uniprot No.

Q96NY8

表达区间及表达系统（Source）

HEK293 Cells-derived Human Nectin-4 Protein, Gly32-Ser349 with hFc tag at the C-terminal

分子量(Molecular Weight)

Approximately 61.1 kDa. Due to glycosylation, the protein migrates to 72-74 kDa based on Tris-Bis PAGE result.

外观(Physical Appearance）

Sterile Filtered White lyophilized (freeze-dried) powder.

纯度（Purity）

> 95% as determined by SDS-PAGE and HPLC

活性（Activity）

SPR Data: Human Nectin-4, hFc Tag captured on Protein A chip, can bind Human Nectin-1,His Tag with an affinity constant of 0.17 μM as determined in a SPR assay (Biacore T200).

内毒素（Endotoxin）

< 1.0 EU per 1μg of the protein by the LAL method.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.

复溶（Reconstitution）

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL` is recommended (usually we use 1mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.