细数不同偶联代次ADC药物纯化工艺

将更加注重提高产品质量、降低成本、适应大规模生产以及满足临床需求；

进一步优化现有纯化技术，开发新的纯化方法；

以及实现多种技术的联用，将是未来ADC药物纯化领域的研究重点。

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[2]. Kos M B P ,,Thomas Raglione Thomas RaglioneChemical Process Development, Bristol Myers Squibb,  Squibb Drive, New Brunswick, New Jersey , United StatesMore by Thomas Raglione,et al.Strategies for UF/DF-Based Impurity Removal in the Post-conjugation Purification of Antibody–Drug Conjugates[J].Organic process research & development, 2023.DOI:10.1021/acs.oprd.3c00137.

[3]. Egbert Müller, Sevilla M , Endres P .Evaluation of hydrophobic‐interaction chromatography resins for purification of antibody‐drug conjugates using a mimetic model with adjustable hydrophobicity[J].Journal of Separation Science, 2020, 43.DOI:10.1002/jssc.201900895.

[4]. Adhikari P, Zacharias N, Ohri R, Sadowsky J. Site-Specific Conjugation to Cys-Engineered THIOMAB? Antibodies. Methods Mol Biol. 2020;2078:51-69. doi:10.1007/978-1-4939-9929-3_4

[5]. Ou J , Si Y , Goh K Y ,et al.Bioprocess development of antibody-drug conjugate production for cancer treatment[J].PLoS ONE, 2018, 13(10).DOI:10.1371/journal.pone.0206246.

[6]. Keller W R , Wendeler M .Using multimodal chromatography for post-conjugation antibody-drug conjugate purification: A methodology from high throughput screening to in-silico process development[J].Journal of Chromatography A, 2021.DOI:10.1016/j.chroma.2021.462378.