Effect of Glutaraldehyde on..Biosensor Preparation

北京盈盛恒泰科技有限责任公司是一家专业从事食品分析检测仪器和环境应急检测仪器的销售技术支持和售后服务工作公司目前是日本INSENT日本JWP意大利VELP美国FTC德国AIRSENSE德国OWR美国UNITY美国Navas美国FiltationEngineering等世界仪器制造商的ZG区营销合作伙伴和技术服务ZX，产品覆盖食品感官分析食品营养分析食品安全检测及环境应急监测等公司在香港北京上海广州成都海口银川等地设有分公司和办事机 The effect of glutaraldehyde on the conformational properties of Kluyveromyces lactis -galactosidase was determined. The optimum substrate of the enzyme interchanged from lactose to sucrose when it reacted with a certain concentration of glutaraldehyde. Its mechanism was studied by detecting the changes in fluorescence absorption. This is the first discovery that -galactosidase can be converted to another kind of enzyme which can catalyze sucrose to produce glucose rather than lactose. On the basis of this phenomenon, an amperometric sucrose biosensor using -galactosidase modified by glutaraldehyde as the immobilized enzyme and poly--cyclodextrin as the carrier was prepared. The response characteristic of the sucrose biosensor was also studied. Under optimum conditions, the current response had a linear relationship with the sucrose concentration in the range of 02 g/dL with a correlation coefficient of 0.9996 and a response time of < 30 s. The sucrose biosensor exhibited excellent diffusion and stability, and retained its sensitivity after 7 d of continuous use. This sucrose biosensor is expected to provide an effective and economical alternative for the quick and sensitive detection of sucrose in the food and beverage industries in the future. 电子鼻-Z传感器生产商产品