Biotinylated Recombinant Human MSLN/Mesothelin (His-Avi Tag)

Uniprot No.

Q13421.2

表达区间及表达系统

Recombinant Biotinylated Human MSLN/Mesothelin Protein is expressed from HEK293 Cells with His tag and Avi tag at the C-terminal. It contains Glu296-Gly580.

分子量

Approximately 35.2 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result.

纯度

> 95% as determined by SDS-PAGE and HPLC.

活性

ELISA Data: Immobilized Biotinylated Human MSLN at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-MSLN Antibody, hFc Tag with the EC50 of 20.3ng/ml determined by ELISA.

内毒素

< 1.0 EU per 1μg of the protein by the LAL method.

制剂

Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.

复溶

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.