epicentre F72250N MasterAmp™ Tfl DNA Pol w/o buffe

• 可选择热稳定的DNA聚合酶进行简化的PCR优化。

• Simplified PCR optimization with a thermostable DNA polymerase of choice.

• 困难或高GC模板的PCR。

• PCR of difficult or high-GC templates.

• PCR扩达20 kb。

• PCR amplifications up to 20 kb.

• Multiplex PCR.

• 多重PCR。

为了快速，可靠地优化PCR，我们建议您尝试使用FailSafe™PCR系统，以不超过20 kb的任何模板获得**PCR结果。但是，如果您更喜欢使用其他酶，EPICENTER还提供了两个MasterAmp™PCR优化套件，这些套件将帮助您使用PCR中常用的热稳定酶（例如Taq，Pfu，Tth，Tfl和AmpliTherm）获得**的PCR结果。™DNA聚合酶。这些MasterAmp PCR优化套件包含一系列12种MasterAmp PCR 2X PreMix，这些产品均经过严格测试，代表了经常遇到的完整PCR条件。每个MasterAmp 2X PreMix包含PCR所需的所有组件，引物，模板和PCR酶除外。

For rapid, dependable optimization of PCR, we encourage you to try the FailSafe™ PCR System to obtain the best PCR results with any template up to ~20 kb. However, if you prefer to use another enzyme, EPICENTRE also has two MasterAmp™ PCR Optimization Kits that will help you to obtain the best possible PCR results with thermostable enzymes frequently used in PCR, such as Taq, Pfu, Tth, Tfl, and AmpliTherm™ DNA polymerases. These MasterAmp PCR Optimization Kits contain a series of 12 MasterAmp PCR 2X PreMixes that have been meticulously tested and represent a complete range of PCR conditions frequently encountered. Each MasterAmp 2X PreMix contains all components needed for PCR except for the primers, the template, and the PCR enzyme.

首先，使用您的模板，引物和12个MasterAmp PreMixes进行PCR。选择提供**结果的PreMix。然后，使用相同的MasterAmp PCR PreMix获得相同序列的可靠，一致的PCR。

First, perform PCR with your template, primers, and the 12 MasterAmp PreMixes. Select the PreMix that gives optimal results. Then, use the same MasterAmp PCR PreMix to obtain reliable, consistent PCR of the same sequence.

好处说明

• 快速，简单的优化。

• Fast, simple optimization.

• Extremely high sensitivity and specificity using the PCR Enhancer Technology.

• 使用PCR增强剂技术具有极高的灵敏度和特异性。

• MasterAmp PCR PreMixes contain all components needed for successful PCR.

• MasterAmp PCR PreMixes包含成功进行PCR所需的所有组件。

• Generates PCR products suitable for both TA cloning and blunt-end cloning.

• 生成同时适用于TA克隆和平末端克隆的PCR产物。