EZ-Tn5™ <oriV /KAN-2> Insertion Kit epicentre EZI0

• Insertion of priming sites and conversion of single-copy clones to multi-copy-inducible clones for higher yields of BAC or fosmid DNA.

• 插入引物位点，并将单拷贝克隆转化为多拷贝诱导型克隆，以提高BAC或fosmid DNA的产量。

The EZ-Tn5™ <oriV/KAN-2> Insertion Kit* randomly inserts a single EZ-Tn5™ <oriV/KAN-2> Transposon containing an inducible, high-copy origin of replication (oriV) into the target DNA. Thus, the kit integrates CopyControl™ capability into those vectors that lack it. TransforMax™ EPI300™ or EPI300™-T1R Electrocompetent E. coli are transformed with an aliquot of the reaction and selected for kanamycin resistance encoded by the EZ-Tn5 Transposon. Selected clones are amplified by adding the CopyControl Induction or Autoinduction Solution, and microgram quantities of template DNA can be purified from as little as 1 ml of culture.

EZ-Tn5™< ori V / KAN-2>插入试剂盒*将包含可诱导的高复制复制起点（ori V）的单个EZ-Tn5™< ori V / KAN-2>转座子随机插入靶标脱氧核糖核酸。因此，该套件将CopyControl™功能集成到了缺乏该功能的载体中。用等分的反应物转化TransforMax™EPI300™或EPI300™-T1 R电感受态大肠杆菌，并选择EZ-Tn5转座子编码的卡那霉素抗性。通过添加CopyControl诱导或自动诱导溶液可扩增选定的克隆，可从低至1 ml的培养物中纯化出微克量的模板DNA。

图例说明

Figure 1 (click to enlarge). The process for generating EZ-Tn5™ <oriV/KAN-2> Transposon insertion clones for high yields of DNA and bidirectional sequencing.

图1（单击放大）。产生EZ-Tn5™< ori V / KAN-2>转座子插入克隆的过程，可实现高产量的DNA和双向测序。