CT1C0210 TransforMax™ EPI300™-T1R Chemically Compe

• 使用CopyControl™克隆系统构建具有可抗污染噬菌体T1和T5的克隆的诱导拷贝数基因组文库。

• Construction of inducible-copy-number genomic libraries using the CopyControl™ Cloning System, with clones that are resistant to contaminating phage T1 and T5.

噬菌体抗T1的TransforMax™EPI300™-T1 R 大肠杆菌  具有功能强大的TransforMax EPI300™ 大肠杆菌  感受态细胞的所有优点，  此外还具有对噬菌体T1和T5（tonA  基因型）的抗性。一旦引入实验室环境，噬菌体T1就会迅速裂解   克隆应用中常用的大肠杆菌菌株，并导致大量的实验室停机时间和有价值的克隆丢失。噬菌体T1特别难以从实验室中清除，并且可以休眠多年。所述  TONA  基因型保护噬菌体TransforMax EC100-T1耐T1- ř  通过噬菌体T1免受攻击细胞和有价值的克隆。

Phage T1-Resistant TransforMax™ EPI300™-T1R E. coli have all the benefits of the highly versatile TransforMax EPI300™ E. coli competent cells with the addition of being resistant to bacteriophages T1 and T5 (tonA genotype). Once introduced into the lab environment, bacteriophage T1 rapidly lyses E. coli strains that are commonly used in cloning applications and results in significant lab downtime and the loss of valuable clones. Bacteriophage T1 is particularly difficult to eliminate from the lab and can lay dormant for many years. The tonA genotype protects the phage T1-resistant TransforMax EC100-T1R cells and valuable clones from attack by bacteriophage T1.

与标准TransforMax EPI300  大肠杆菌一样，该菌株经过特殊设计，可与EPICENTRE的CopyControl™克隆系统一起使用。*细胞含有可诱导的突变  trfA  基因，其基因产物是从CopyControl上的ori V 起始复制所需  的pCC1™载体或使用EZ-Tn5™< ori V / KAN-2>插入套件通过CopyControl功能改造的克隆。该过程使TransforMax EPI300-T1 R.  coli  能够将CopyControl载体保持为单拷贝，直到在DNA纯化前立即进行诱导。

Like the standard TransforMax EPI300 E. coli, this strain has been specially engineered for use with EPICENTRE's CopyControl™ Cloning Systems.* The cells contain an inducible mutant trfA gene whose gene product is required for initiation of replication from the oriV contained on the CopyControl pCC1™ Vectors or on clones that have been retrofitted with CopyControl capability using the EZ-Tn5™<oriV/KAN-2> Insertion Kit. This process allows TransforMax EPI300-T1R E. coli to maintain CopyControl vectors at single copy until induction occurs immediately before DNA purification.

优势说明

• tonA  用于抵抗噬菌体T1和T5。

• trfA  基因受可诱导启动子的严格控制，用于CopyControl克隆和EZ-Tn5 ori V / KAN-2转座子改造的克隆的拷贝数控制。

• 大型和小型克隆的转化效率都很高。

• lacZΔM15  用于重组体的蓝/白筛选。

• tonA for resistance to bacteriophages T1 and T5.

• trfA gene under tight control of an inducible promoter for copy-number control of CopyControl clones and clones retrofitted with the EZ-Tn5<oriV/KAN-2> Transposon.

• High transformation efficiency of both large and small clones.

• lacZΔM15 for blue/white screening of recombinants.

• Readily accepts large DNAs for construction of large-insert genomic libraries.

• Restriction minus [mcrA, Δ(mrr-hsdRMS-mcrBC)] enables efficient cloning of methylated DNA.

• Endonuclease minus (endA1) to ensure high yields of DNA.

• Recombination minus (recA1) for greater stability of large cloned inserts.

• 随时接受大DNA用于构建大插入基因组文库。

• 限制酶 [ mcrA，Δ（mrr-hsdRMS-mcrBC） ]可以使甲基化DNA高效克隆。

• 核酸内切酶负号（endA1）可确保高产量的DNA。

• 重组减号（recA1）可提高大型克隆插入片段的稳定性。

基因型

˚F -  MCRA d（MRR-hsdRMS-的McrBC）F80dlacZDM15 DlacX74 recA1 endA1 araD139 d（ARA，LEU）7697嘎鲁的galK升-  的rpsL（力量- [R）nupG TRFA TONA

F- mcrA Δ(mrr-hsdRMS-mcrBC) Φ80dlacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara, leu)7697 galU galK λ- rpsL (StrR) nupG trfA tonA

*由已发布和/或正在申请的ZG覆盖。

CT1C0210 TransforMax™ EPI300™-T1R Chemically Competent E.coli  货号说明