This application note looks at the principles of manipulating
developed methods to get shorter analysis times by
making alterations that affect speed, but not the quality of
the chromatography. As an illustration, a method for a
standard doping drugs test mixture was developed using a
0.25 mm Thermo Scientific ID TRACE™ TR-5MS column.
This column gave good separation of nine compounds in
around 22 minutes for the original analysis. The method
was adapted to a shorter column, maintaining the chromatography
but reducing the time required for analysis.
The analysis was carried out using a Thermo Scientific
DSQ™ GC/MS system in conjunction with TRACE
TR-5MS GC columns.
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